Developing Bio-Based Self-Healing Concrete: An Eco-Friendly Strategy for Sustainability of Concrete

Document Type : Research Note

Authors

1 Research Scholar (Ph.D.), in Dept. of Civil Engineering, Andhra University, Visakhapatnam, India

2 Prof. in Dept. of Civil Engineering, College of Engineering, Andhra University, Visakhapatnam, India

Abstract

There is a growing global demand for concrete, making it crucial to develop sustainable concrete solutions that address environmental concerns. Currently, approximately 7% of total human-caused CO2 emissions in the atmosphere come from cement production material. Techniques that incorporate bacteria to increase the lifespan of concrete elements will not only enhance their longevity but also contribute to more sustainable concrete materials for future generations. In this research, four different proportions of bacteria (0.5%, 1.5%, 3.5%, and 5%) and nutrients (0.10%, 0.30%, 0.5%, and 0.75%) were used relative to the weight of the cement. The objective of this research is to assess the capacity of bacteria to function as self-repairing agents in concrete elements, specifically their ability to fix cracks by producing spores that form within the concrete. The spores of the bacteria mixed directly into the concrete mixture remained viable for an extended period. The gradual reduction of pore diameter during the concrete setting likely limited the longevity and reproduction of spores, as the pore widths were reduced to 1µm or less. However, concrete mixed with bacteria exhibited a significantly higher production of minerals that fill cracks compared to normal concrete specimens. At various time intervals, the samples were tested for their compressive strength and microstructural characteristics using EDX, SEM, and XRD. The most significant improvement occurred at 28 days, with proportions of 1.5% bacteria and 0.3% calcium lactate relative to the weight of the cement, resulting in a 27.1% increase in compressive strength and the healing of cracks measuring between 1 and 3 mm. This suggests that the use of bacterial spores as a self-sealing agent holds enormous potential.

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References

[1]      Long AE, Henderson GD, Montgomery FR. Why assess the properties of near-surface concrete? Constr Build Mater 2001. https://doi.org/10.1016/S0950-0618(00)00056-8.
[2]      Neville A. Consideration of durability of concrete structures: Past, present, and future. Mater Struct 2001. https://doi.org/10.1007/bf02481560.
[3]      Basheer L, Kropp J, Cleland DJ. Assessment of the durability of concrete from its permeation properties: A review. Constr Build Mater 2001. https://doi.org/10.1016/S0950-0618(00)00058-1.
[4]      Pan X, Shi Z, Shi C, Ling TC, Li N. A review on surface treatment for concrete – Part 2: Performance. Constr Build Mater 2017. https://doi.org/10.1016/j.conbuildmat.2016.11.128.
[5]      Mithanthaya IR, Shet VB, Mokshitha M. Mechanism of healing, strength, and durability concepts of bacteria concrete - A review. IOP Conf Ser Earth Environ Sci 2024;1387. https://doi.org/10.1088/1755-1315/1387/1/012003.
[6]      Wang J, Ji S, Huang S, Jiang Z, Wang S, Zhang H, et al. Crack Sealing in Concrete with Biogrout: Sustainable Approach to Enhancing Mechanical Strength and Water Resistance. Materials (Basel) 2024;17:1–17. https://doi.org/10.3390/ma17246283.
[7]      Jiang L, Li P, Wang W, Zhang Y, Li Z. A self-healing method for concrete cracks based on microbial-induced carbonate precipitation: bacteria, immobilization, characterization, and application. J Sustain Cem Mater 2024. https://doi.org/10.1080/21650373.2023.2263447.
[8]      Folić R, Zenunović D, Brujić Z. Effects of carbonation and chloride ingress on the durability of concrete structures. J Serbian Chem Soc 2024;89:729–42. https://doi.org/10.2298/JSC240102030F.
[9]      Mutitu DK, Muthengia JW, Mwirichia R, Thiong’o JK, Mulwa MO, Genson M. Microbial effect on water sorptivity and sulphate ingress by Bacillus megaterium on mortars prepared using Portland Pozzolana cement. J Appl Microbiol 2021. https://doi.org/10.1111/jam.14976.
[10]     Osta MO, Mukhtar F. Effect of bacteria on uncracked concrete mechanical properties correlated with damage self-healing efficiency – A critical review. Dev Built Environ 2024. https://doi.org/10.1016/j.dibe.2023.100301.
[11]     Kaushal V, Saeed E. Sustainable and Innovative Self-Healing Concrete Technologies to Mitigate Environmental Impacts in Construction. CivilEng 2024;5:549–58. https://doi.org/10.3390/civileng5030029.
[12]     Gerilla GP, Teknomo K, Hokao K. An environmental assessment of wood and steel reinforced concrete housing construction. Build Environ 2007. https://doi.org/10.1016/j.buildenv.2006.07.021.
[13]     Chen L, Nouri Y, Allahyarsharahi N, Naderpour H, Rezazadeh Eidgahee D, Fakharian P. Optimizing compressive strength prediction in eco-friendly recycled concrete via artificial intelligence models. Multiscale Multidiscip Model Exp Des 2025;8:24. https://doi.org/10.1007/s41939-024-00641-x.
[14]     Koch. Corrosion costs and preventive strategies in the United States. US Federal Highway Administration. Mater Perform 2002.
[15]     Day KW. Properties of concrete. Concr. Mix Des. Qual. Control Specif., 2021. https://doi.org/10.4324/9780203967874-11.
[16]     Infrastructure W. Public spending on transportation and water infrastructure. Transp Water Infrastruct Spend 2023;2017:1–66.
[17]     Brent P, Bridge S, Project C. 2025: c 2021: c 2025.
[18]     Mohamed A, Fan M, Bertolesi E, Chen H, Fu Z, Roberts T. Microbial loading and self-healing in cementitious materials: A review of immobilisation techniques and materials. Mater Des 2024;245:113249. https://doi.org/10.1016/j.matdes.2024.113249.
[19]     Huang H, Ye G, Qian C, Schlangen E. Self-healing in cementitious materials: Materials, methods and service conditions. Mater Des 2016. https://doi.org/10.1016/j.matdes.2015.12.091.
[20]     Sun J. Open Aircraft Performance Modeling Based on an Analysis of Aircraft Surveillance Data. 2019. https://doi.org/10.4233/uuid.
[21]     Kim TK, Park JS. Experimental evaluation of the durability of concrete repair materials. Appl Sci 2021. https://doi.org/10.3390/app11052303.
[22]     A.M. Neville. Concrete technology Second edition. United Kingdom, Prentice Hall 2010.
[23]     Neville AM. Properties of concrete-5th edition. 2011.
[24]     Anum I, Williams F., Adole A., Haruna A. Properties of Different Grades of Concrete Using Mix Design Method. Int J Geol Agric Environ Sci 2014.
[25]     Provis JL, Bílek V, Buchwald A, Dombrowski-Daube K, Varela B. Durability and testing – Physical processes. RILEM State-of-the-Art Reports 2014. https://doi.org/10.1007/978-94-007-7672-2_10.
[26]     Cabral AR, Moreira JF V., Jugnia L-B. Biocover Performance of Landfill Methane Oxidation: Experimental Results. J Environ Eng 2010. https://doi.org/10.1061/(asce)ee.1943-7870.0000182.
[27]     Al-Heetimi OT, Van De Ven CJC, Van Geel PJ, Rayhani MT. Impact of temperature on the performance of compost-based landfill biocovers. J Environ Manage 2023. https://doi.org/10.1016/j.jenvman.2023.118780.
[28]     Cheng L, Shahin MA, Mujah D. Influence of Key Environmental Conditions on Microbially Induced Cementation for Soil Stabilization. J Geotech Geoenvironmental Eng 2017. https://doi.org/10.1061/(asce)gt.1943-5606.0001586.
[29]     Rodriguez-Navarro C, Rodriguez-Gallego M, Chekroun K Ben, Gonzalez-Muñoz MT. Conservation of ornamental stone by Myxococcus xanthus-induced carbonate biomineralization. Appl Environ Microbiol 2003. https://doi.org/10.1128/AEM.69.4.2182-2193.2003.
[30]     Jimenez-Lopez C, Jroundi F, Rodriguez-Gallego M, Arias JM, González-Muñoz MT. Biomineralization induced by Myxobacteria. Commun. Curr. Res. Educ. Top. Trends Appl. Microbiol., 2007.
[31]     Wang R, Qian C, Wang J. Study on microbiological precipitation of CaCO3. Dongnan Daxue Xuebao (Ziran Kexue Ban)/Journal Southeast Univ (Natural Sci Ed 2005.
[32]     Cheng L, Shahin MA. Microbially Induced Calcite Precipitation (MICP) for Soil Stabilization, 2019. https://doi.org/10.1007/978-981-13-0149-0_3.
[33]     Zhang N, Fu Q, Wang J, Lu L, Luo Q, Xing F. Evaluation of compressive strength and chloride permeability of cement-based materials with high-volume compound mineral admixtures. Adv Cem Res 2024. https://doi.org/10.1680/jadcr.23.00185.
[34]     Islam MM, Hoque N, Islam M, Ibney Gias I. An Experimental Study on the Strength and Crack Healing Performance of E. coli Bacteria-Induced Microbial Concrete. Adv Civ Eng 2022. https://doi.org/10.1155/2022/3060230.
[35]     Kumar Jogi P, Vara Lakshmi TVS. Self healing concrete based on different bacteria: A review. Mater. Today Proc., 2020. https://doi.org/10.1016/j.matpr.2020.08.765.
[36]     Bang SS, Galinat JK, Ramakrishnan V. Calcite precipitation induced by polyurethane-immobilized Bacillus pasteurii. Enzyme Microb Technol 2001. https://doi.org/10.1016/S0141-0229(00)00348-3.
[37]     Piras S, Salathia S, Guzzini A, Zovi A, Jackson S, Smirnov A, et al. Biomimetic Use of Food-Waste Sources of Calcium Carbonate and Phosphate for Sustainable Materials—A Review. Materials (Basel) 2024. https://doi.org/10.3390/ma17040843.
[38]     Javeed Y, Goh Y, Mo KH, Yap SP, Leo BF. Microbial self-healing in concrete: A comprehensive exploration of bacterial viability, implementation techniques, and mechanical properties. J Mater Res Technol 2024. https://doi.org/10.1016/j.jmrt.2024.01.261.
[39]     British Standards Institution BSI. BS EN 450-1:2012: Fly ash for concrete - Part 1: Definition, specifications and conformity criteria. Br Stand 2012.
[40]     van Raaij WF. The Use of Natural Resources. Handb Econ Psychol 2018:638–55. https://doi.org/10.1007/978-94-015-7791-5_18.
[41]     Kiersma ME. Occupational Safety and Health Administration. Encycl. Toxicol. Third Ed., 2014. https://doi.org/10.1016/B978-0-12-386454-3.00344-4.
[42]     Zhang Y, Li J, Liu F, Yan H, Li J. Mediative mechanism of bicarbonate on anaerobic propionate degradation revealed by microbial community and thermodynamics. Environ Sci Pollut Res 2018. https://doi.org/10.1007/s11356-018-1430-7.
[43]     EC. Directive 2000/60/EC of the European Parliament and of the Council of 23 October 2000 establishing a framework for Community action in the field of water policy. Off J Eur Parliam 2000. https://doi.org/10.1039/ap9842100196.
[44]     Anon. Aquatic Life Ambient Water Quality Criteria for Ammonia – Freshwater. United States Environ Prot Agency 2013.
[45]     Program T. ASCP 7 TH CONCRETE PAVEMENTS CONFERENCE PAVING THE FUTURE TECHNICAL PROGRAM 2023.
[46]     Jonkers HM, Thijssen A, Muyzer G, Copuroglu O, Schlangen E. Application of bacteria as self-healing agent for the development of sustainable concrete. Ecol Eng 2010. https://doi.org/10.1016/j.ecoleng.2008.12.036.
[47]     Jonkers HM, Schlangen E. Development of a bacteria-based self healing concrete. Proc. Int. FIB Symp. 2008 - Tailor Made Concr. Struct. New Solut. our Soc., 2008. https://doi.org/10.1201/9781439828410.ch72.
[48]     Murari K, Kaur P. Development of Sustainable Concrete Using Bacteria as Self-healing Agent. Lect. Notes Civ. Eng., 2021. https://doi.org/10.1007/978-981-15-9554-7_62.
[49]     Rao MVS, Reddy VS, Hafsa M, Veena P, Anusha P. Bioengineered concrete - A sustainable self-healing construction material. Res J Eng Sci 2013.
[50]     Nimafar M, Samali B, Hosseini SJ, Akhlaghi A. Use of bacteria externally for repairing cracks and improving properties of concrete exposed to high temperatures. Crystals 2021. https://doi.org/10.3390/cryst11121503.
[51]     Wang J, Ersan YC, Boon N, De Belie N. Application of microorganisms in concrete: a promising sustainable strategy to improve concrete durability. Appl Microbiol Biotechnol 2016. https://doi.org/10.1007/s00253-016-7370-6.
[52]     Tang H, Li XG. Research on autogenous healing of cracks in cement based materials. Wuhan Ligong Daxue Xuebao/Journal Wuhan Univ Technol 2008.
[53]     Van Tittelboom K, De Belie N, De Muynck W, Verstraete W. Use of bacteria to repair cracks in concrete. Cem Concr Res 2010. https://doi.org/10.1016/j.cemconres.2009.08.025.
[54]     Mors RM, Jonkers HM. Bacteria‐based self‐healing concrete: Evaluation of full scale demonstrator projects. RILEM Tech Lett 2019. https://doi.org/10.21809/rilemtechlett.2019.93.
[55]     Wiktor V, Jonkers HM. Quantification of crack-healing in novel bacteria-based self-healing concrete. Cem Concr Compos 2011. https://doi.org/10.1016/j.cemconcomp.2011.03.012.
[56]     Stieber M, Haeseler F, Werner P, Frimmel FH. A rapid screening method for micro-organisms degrading polycyclic aromatic hydrocarbons in microplates. Appl Microbiol Biotechnol 1994. https://doi.org/10.1007/BF00173340.
[57]     Zhao H, Xiao Q, Huang D, Zhang S. Influence of pore structure on compressive strength of cement mortar. Sci World J 2014. https://doi.org/10.1155/2014/247058.
[58]     Jiang G, Zhou M, Chiu TH, Sun X, Keller J, Bond PL. Wastewater-Enhanced Microbial Corrosion of Concrete Sewers. Environ Sci Technol 2016. https://doi.org/10.1021/acs.est.6b02093.
[59]     Newman EB. General microbiology. Res Microbiol 1994. https://doi.org/10.1016/0923-2508(94)90009-4.

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History

  • Receive Date: 24 March 2025
  • Revise Date: 15 May 2025
  • Accept Date: 03 July 2025

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